AWS HealthOmics now calculates Entity Tags (ETags) when ingesting into the sequence store. Sequence store ETags enable customers to validate that files are not altered as part of their ingestion into, storage lifecycle in, and egress from the sequence store, an important audit requirement for many clinical and life science users. The new capability calculates a hash of the file’s semantic content during sequence store imports and direct uploads, making it easier to identify when content is changed or duplicated. The ETag’s immutability, verifiability, and auto-calculation simplifies data audits, duplicate data identification, and supports customer compliance validation.